The determination of catechol amines in blood.
نویسندگان
چکیده
1HE GROWING CLINICAL interest in the action and metabolism of the catechol amines makes it highly desirable to re-evaluate existing methods for the quantitative determination of these substances in body fluids. In 1955 Persky (1) reviewed the subject, and, on the whole, his conclusion is still valid that the fluorometric determination of the catechol amines in body fluids is the most practical method for clinical purposes. For the determination of the two catechol amines in the presence of each other, the methods of Lunt (2, 3) and of Weil-Maiherbe (4), and modifications thereof, are used most frequently. The former depends upon the differential oxidation of the catechol amines at pH 3.5 and 6.5 and the anaerobic rearrangements of the resulting o-quinones to the intensively fluorescing “lutin” derivatives. In the Weil-Maiherbe method the fluorescence is stabilized by coupling the quinones in the status nascendi with ethylene diamine, and measuring the emitted fluorescence at two different wave lengths. According to Persky (1), methods depending upon the ethylene diamine condensation are somewhat more reliable than those involving oxidation at different pH values. However, the precision of this method depends to a large extent on the assumed constancy of the fluorescence in the region between 510 and 600 mjL (5). Recently Goldfien and Karler (6) demonstrated the effect of light on the fading of the fluorescence of the ethylene diamine condensation products of the oxidized catechol amines. Studies carried out in this laboratory during the last year fully confirm these results
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 5 3 شماره
صفحات -
تاریخ انتشار 1959